replication fork is initiated at the origin and then moves sequentially along DNA

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#Unit 3. Fundamental Processes

DnaA promotes replication

SeqA delays new round of replication by inhibiting dam methylase binding

Dam methylase provide substrate for DnaA

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#Unit 3. Fundamental Processes

DnaA mutants make replication asynchronous

SeqA mutant cells don’t delay rereplication for 13 minutes

Dam methylase mutants stops rereplication

SeqA-/dam+ accumulation of methylated products

SeqA+/dam- accumulation of hemimethylated products

SeqA-/dam- accumulation of hemimethylated products

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#Unit 3. Fundamental Processes

There are two basic types of DNA synthesis

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#Unit 3. Fundamental Processes

Semiconservative replication

DNA repair reaction

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#Unit 3. Fundamental Processes

All prokaryotic and eukaryotic DNA polymerases share the same fundamental type of synthetic activity -an antiparallel synthesis from 5’ to 3’ from a template that is 3’ to 5’. This means adding nucleotides one at a time to a 3’–OH end.

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#Unit 3. Fundamental Processes

Hydrolysis of pyrophosphate is the driving force for DNA synthesis