FBPase-1 is a gluconeogenic enzyme

TLS Online TPP Program

#Id: 860

#Unit 13. Methods in Biology

The PCR consists of three defined sets of times and temperatures, termed steps

a) Denaturation at 94°C, for 0.5 min

b) Primer annealing at 55°C for 1.5 min

c) Extension at 72°C for 1 min

TLS Online TPP Program

#Id: 861

#Unit 13. Methods in Biology

The time taken for each cycle is considerably longer than 3 min (0.5 + 1.5 +1 min)

TLS Online TPP Program

#Id: 862

#Unit 13. Methods in Biology

Polymerase chain reaction is based on thermal modulation and the only major difference in PCR and Replication is Origin Site.

TLS Online TPP Program

#Id: 863

#Unit 13. Methods in Biology

Thermal modulations are used in PCR for the functions of helicase and polymerase.

TLS Online TPP Program

#Id: 864

#Unit 13. Methods in Biology

In the PCR denaturation temperature calculated by using the Wallace rule, which is, Tm = 4 × (number of GC base pairs) + 2 × (number of AT base pairs).

TLS Online TPP Program

#Id: 865

#Unit 13. Methods in Biology

The size of a PCR product is mainly determined by the binding location of the PCR primers.