In Shuttle reaction the 3' oxygen extracts a hydrogen from the 2'-hydroxyl, and the 2' oxygen, in turn, extracts a hydrogen from the amino group attacking the carbonyl.

TLS Online TPP Program

#Id: 8302

#Unit 1. Molecules and their Interaction Relevant to Biology

TLS Online TPP Program

#Id: 8309

#Unit 1. Molecules and their Interaction Relevant to Biology

If weight of enzyme is given use molar conversion (wt/mol. Wt)

TLS Online TPP Program

#Id: 8310

#Unit 1. Molecules and their Interaction Relevant to Biology

TLS Online TPP Program

#Id: 8541

#Unit 1. Molecules and their Interaction Relevant to Biology

The dependent variable is the rate of the reaction. 

The independent variables are the concentration of enzyme, the concentration of the substrate, the temperature, and the addition of an inhibitor

TLS Online TPP Program

#Id: 8542

#Unit 1. Molecules and their Interaction Relevant to Biology

If the concentration of the enzyme or substrate is increased, the rate of the reaction will be increased as well. 

If the temperature of the enzymes surrounding environment is increased, then the rate of the reaction will also be increased. 

If the substrate concentration is increased then the rate of the reaction will increase. 

If an inhibitor is added then the rate of the reaction will be decreased significantly

TLS Online TPP Program

#Id: 8543

#Unit 1. Molecules and their Interaction Relevant to Biology

One drawback from the Eadie–Hofstee approach is that neither ordinate nor abscissa represent independent variables: both are dependent on reaction rate. Thus any experimental error will be present in both axes.