In a natural closed DNA that is negatively supercoiled, the intercalation of ethidium bromide first removes the negative supercoils and then introduces positive supercoils.

TLS Online TPP Program

#Id: 2913

#Unit 3. Fundamental Processes

Gyrase, a type II topoisomerase, provides a swivel that allows one DNA strand to rotate around the other.

TLS Online TPP Program

#Id: 2914

#Unit 3. Fundamental Processes

The protein single-strand binding protein (SSB) stabilizes the single-stranded DNA as it is formed and modulates the helicase activity.

TLS Online TPP Program

#Id: 2915

#Unit 3. Fundamental Processes

The length of duplex DNA that usually is unwound to initiate replicati011 is probably <60 bp.

TLS Online TPP Program

#Id: 2916

#Unit 3. Fundamental Processes

HU has the capacity to bend DNA, and is involved in building the structure that leads to formation of the open complex.

TLS Online TPP Program

#Id: 2917

#Unit 3. Fundamental Processes

Input of energy in the form of ATP is required at several stages for the prepriming reaction, and it is required for unwinding DNA.

TLS Online TPP Program

#Id: 2918

#Unit 3. Fundamental Processes

Once the prepriming complex is loaded onto the replication forks, the next step is the recruitment of the primase, DnaG, which is then loaded onto the DnaB hexamer.