The best method for cryopreserving cultured cells is storing them in liquid nitrogen in complete medium in the presence of a cryoprotective agent such as dimethylsulfoxide (DMSO) or glycerol

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#Id: 170

#Unit 5. Developmental Biology

BMP4 

A protein that is received by the lens cells and induces the production of the Sox transcription factors.

TLS Online TPP Program

#Id: 171

#Unit 5. Developmental Biology

FGF8 

A secreted signal that is received by the lens cells and induces the production of the L-Maf transcription factor.

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#Id: 172

#Unit 5. Developmental Biology

Combination of Pax6, Sox2, and L-Maf in the ectoderm is needed for the production of the lens and the activation of lens-specific genes i.e. δ-crystallin.

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#Id: 173

#Unit 5. Developmental Biology

If Pax6 is lost (fruit flies, frogs, rats, humans) complete loss or reduction of the eyes

TLS Online TPP Program

#Id: 174

#Unit 5. Developmental Biology

Two major modes of inductive interaction:

 Instructive interaction

 Permissive interaction

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#Id: 175

#Unit 5. Developmental Biology

In instructive interaction, 

a signal from the inducing cell is necessary for initiating new gene expression in the responding cell.