Monoubiquitinylation of the HER1 cytosolic domain by the E3 ubiquitin ligase c-Cbl

TLS Online TPP Program

#Id: 7911

#Unit 2. Cellular Organization

MCSs also serve as interfaces for the transfer of ions (Ca+2 ) and small molecules (glycerophospholipids) between the ER and mitochondria.

TLS Online TPP Program

#Id: 7912

#Unit 2. Cellular Organization

The majority of proteins located in mitochondria are not encoded by mtDNA, they are encoded by genes in the nucleus and are imported into the mitochondria after their synthesis in the cytosol.

TLS Online TPP Program

#Id: 7913

#Unit 2. Cellular Organization

Matrix-targeting sequences

sequences for localizing proteins to mitochondria

TLS Online TPP Program

#Id: 7914

#Unit 2. Cellular Organization

Matrix-targeting sequences

Located at the N-terminus

Usually 20–50 amino acids in length

Rich in hydrophobic amino acids, positively charged basic amino acids (arginine & lysine), and hydroxylated ones (serine and threonine)

Amphipathicity of matrix-targeting sequences is critical to their function

TLS Online TPP Program

#Id: 7915

#Unit 2. Cellular Organization

Mitochondrial protein import requires: 

Tom proteins (Tom 20/22/70)

General import pore (Tom40)

Tim proteins (Tim23/17)

TLS Online TPP Program

#Id: 7916

#Unit 2. Cellular Organization

Energy inputs are needed to Import proteins into mitochondria

Chaperone proteins (Hsp70& Hsp90)

An H+ electrochemical gradient, or proton-motive force