Flow cytometry or FACS is the sine qua non (without which, nothing) of the modern immunologist’s toolbox.

Flow cytometry is based on light scattering and fluorescence 

Forward light scattering detect shape and side scattering detects granularity and complexity of the cell

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#Unit 2. Cellular Organization

In interphase nuclei, this underlying proteinaceous structure changes its organization to occupy the entire nucleus; during interphase the structure is referred to as the matrix rather than the scaffold.

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#Unit 2. Cellular Organization

S/MARs is a DNA sequence which binds to base structure called scaffold.

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#Unit 2. Cellular Organization

If histones are depleted from a metaphase chromosome and viewed under a transmission electron microscope shows SCAFFOLD.

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#Unit 2. Cellular Organization

The metaphase scaffold consists of a dense network of fibers. Threads of DNA emanate from the scaffold, apparently as loops of average length 30 to 90 kb.

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#Unit 2. Cellular Organization

S/MARs are constitutive (continuously bound to the matrix or scaffold), or facultative and change their interactions with the matrix depending on cell type or other conditions.

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#Unit 2. Cellular Organization

SATB1, is a protein that appears to regulate the association of MARs with the matrix in order to regulate transcription of genes in the vicinity of the MAR.