A number of amino acids (Asp, Glu, Asn, Gln, Arg and His) have weak electronic transitions at around 210 nm. Usually, these cannot be observed in proteins because

they are masked by the more intense peptide bond absorption.

TLS Online TPP Program

#Id: 2892

#Unit 3. Fundamental Processes

DnaA is an ATP-binding protein. The first stage in initiation is binding of the DnaA-ATP 

protein complex to the fully methylated oriC sequence.

TLS Online TPP Program

#Id: 2893

#Unit 3. Fundamental Processes

DnaA is in the active form only when bound to ATP.

TLS Online TPP Program

#Id: 2894

#Unit 3. Fundamental Processes

Dna A has intrinsic ATPase activity that hydrolyzes ATP to ADP and thus inactivates itself when the initiation stage ends.

TLS Online TPP Program

#Id: 2895

#Unit 3. Fundamental Processes

This ATPase activity of Dna A is stimulated by membrane phospholipids and single-stranded DNA.

TLS Online TPP Program

#Id: 2897

#Unit 3. Fundamental Processes

The origin of the replication region remains attached to the membrane for about one-third of the cell cycle as another part of the mechanism to prevent reinitiation.

TLS Online TPP Program

#Id: 2899

#Unit 3. Fundamental Processes

Opening oriC involves action at two types of sequence in the origin: 9-bp and 13 -bp repeats.