Biochemical samples are usually buffered aqueous solutions, which has two major advantages. Firstly, proteins and peptides are comfortable in water as a solvent, which is also the ‘native’ solvent. Secondly, in the wavelength interval of UV/Vis (700–200 nm) the water spectrum does not show any absorption bands and thus acts as a silent component of the sample
#Unit 13. Methods in Biology#UV/visible#Part B Pointers