Biochemical samples are usually buffered aqueous solutions, which has two major advantages. Firstly, proteins and peptides are comfortable in water as a solvent, which is also the ‘native’ solvent. Secondly, in the wavelength interval of UV/Vis (700–200 nm) the water spectrum does not show any absorption bands and thus acts as a silent component of the sample

TLS Online TPP Program

#Id: 2923

#Unit 3. Fundamental Processes

Seq A binds to hemi methylated DNA and is required for delaying rereplication. 

TLS Online TPP Program

#Id: 2924

#Unit 3. Fundamental Processes

SeqA may interact with DnaA.

TLS Online TPP Program

#Id: 2925

#Unit 3. Fundamental Processes

As the origins are hemimethylated they bind to the cell membrane and may be unavailable to methylases. 

TLS Online TPP Program

#Id: 2926

#Unit 3. Fundamental Processes

As the origins are hemimethylated they bind to the cell membrane and may be unavailable to methylases. 

TLS Online TPP Program

#Id: 2930

#Unit 3. Fundamental Processes

The dat locus contains DnaA-binding sites that titrate availability of DnaA protein.

TLS Online TPP Program

#Id: 2931

#Unit 3. Fundamental Processes

Hda protein is recruited to the replication origin to convert DnaA-ATP to DnaA-ADP.