TLS Online TPP Program

#Id: 4723


The absorption spectrum of a chromophore is only partly determined by its chemical structure. The environment also affects the observed spectrum, which mainly can
be described by three parameters:

protonation/deprotonation (pH, RedOx);
• solvent polarity (dielectric constant of the solvent); and
• orientation effects.

#Unit 13. Methods in Biology #UV/visible #Part B Pointers