RNA gives absorbance at 595 nm in presence of Diphenylamine while gives directabsorbance at 540 nm.

TLS Online TPP Program

#Id: 6223

#Unit 12. Applied Biology

TLS Online TPP Program

#Id: 2733

#Unit 12. Applied Biology

For the generation of transgenic plant in crop improvement, one important regulatory gene ‘X’ was overexpressed in a crop plant. Out of 30 transgenic rice plants generated, 22 showed high levels of gene ‘X’ expression. However, rest 8 lines displayed low level of expression. One explanation of such observation may be: 

Suppression effect of the transgene 

Knock down effect of gene X 

Gene silencing effect 

Co-suppression effect of transgene

 

TLS Online TPP Program

#Id: 6668

#Unit 3. Fundamental Processes

Two transesterification reactions takes place for 2 exon -1 intron configuration. First transesterification is catalysed variably either by external G or branch point while second transesterification is always caused by exon 1 -3’OH.

TLS Online TPP Program

#Id: 878

#Unit 13. Methods in Biology

The efficiency of FRET is inversely proportional to the sixth power of the distance between donor and acceptor.

TLS Online TPP Program

#Id: 583

#Unit 2. Cellular Organization

CAP–cAMP binds upstream to promoters DNA and target bends the DNA considerably about 100 degrees