Activation of the M2 receptors decreases cyclic adenosine 3′,5′-monophosphate (cAMP) in the cells, and this slows the opening of Ca 2+ channels.

TLS Online TPP Program

#Id: 6453

#Unit 3. Fundamental Processes

Liquid chromatography separates and identifies the three eukaryotic RNA polymerases, each with its own sensitivity to 𝛂-amanitin.

TLS Online TPP Program

#Id: 6454

#Unit 3. Fundamental Processes

The α-amanitin binding site is beneath a “bridge helix” extending across the cleft between the two largest pol II subunits, Rpb1 and Rpb2

a DNA template, whereas in eukaryotes, transcription takes place on a chromatin template.

TLS Online TPP Program

#Id: 6455

#Unit 3. Fundamental Processes

Eukaryotic nuclei contain RNA polymerase I, RNA polymerase II, and RNA polymerase III can be separated by using DEAE-Sephadex ion-exchange chromatography

TLS Online TPP Program

#Id: 6456

#Unit 3. Fundamental Processes

RNA Polymerase can also separated by using epitope tagging. Normally RPB3 is used to create epitope tag.

TLS Online TPP Program

#Id: 6457

#Unit 3. Fundamental Processes

Molecular genetic techniques are used to make a cDNA encoding a recombinant protein to which is fused a short sequence of amino acids called an epitope tagging.

TLS Online TPP Program

#Id: 6458

#Unit 3. Fundamental Processes

Two commonly used epitope tags are FLAG, which encodes the amino acid sequence DYKDDDDK (single-letter amino acid code), and myc, which encodes the sequence EQKLISEEDL.