Pluripotency A stem cell that has the potential to differentiate into any of three germ layers(endoderm , mesoderm & ectoderm). Eg-all embryonic cells

TLS Online TPP Program

#Id: 425

#Unit 2. Cellular Organization

This 30-nm fiber is further folded, results in 1000-fold compaction in euchromatin in interphase chromatin and in mitotic chromosomes to achieve an overall 10,000- fold compaction in euchromatin.

TLS Online TPP Program

#Id: 426

#Unit 2. Cellular Organization

Heterochromatin generally maintains 10,000-fold compaction in both interphase and mitosis after 30 nm.

TLS Online TPP Program

#Id: 427

#Unit 2. Cellular Organization

Chromatin contains DNA, Histones, Nonhistone Proteins and RNA. RNA polymerase is a prominent nonhistone protein

TLS Online TPP Program

#Id: 428

#Unit 2. Cellular Organization

Individual nucleosomes can be obtained by treating chromatin with the endonuclease micrococcal nuclease (MNase), which cuts the DNA between nucleosomes, a region known as linker DNA.

TLS Online TPP Program

#Id: 429

#Unit 2. Cellular Organization

Mononucleosomes typically have 200 bp DNA. End-trimming reduces the length of DNA first to 165 bp and then generates core particles with 146 bp.

TLS Online TPP Program

#Id: 430

#Unit 2. Cellular Organization

Mnase digestion result on centrifugation and electrophoresis