Nurturing Life Sciences
#Id: 5850
Island biogeography
#Id: 2599
#Unit 13. Methods in Biology
TaqMan assay also applicable in analyzing variations in the allele or deletion
of base substitution.
#Id: 2600
Touch down PCR are derived to enhance primer binding in the annealing step.
#Id: 2601
A modification of overlap extension PCR may also be used to construct directed mutations; this is termed megaprimer PCR .
#Id: 2602
Long primers forms most probably dimers, so taq pol amplifying this (in low temp) also.
#Id: 2603
The SNP mutation responsible for sickle-cell disease changes amino acid 6 in haemoglobin β from glutamic acid (E) to valine (V).
#Id: 2604
The sequence of the PCR product from a heterozygous carrier shows both the normal (HbβA) and mutant (HbβS) nucleotides at the position of the substitution SSR genotyping by PCR product electrophoresis.
