Hydrolysis of pyrophosphate is the driving force for DNA synthesis

TLS Online TPP Program

#Id: 6676

#Unit 3. Fundamental Processes

Prp5 is critical for U2 binding

Brr2 facilitates U1 and U4 release

Prp2 is responsible for the activation of the spliceosome Prp22 helps the release of the mature mRNA from the spliceosome

TLS Online TPP Program

#Id: 6677

#Unit 3. Fundamental Processes

Prp22 helps the release of the mature mRNA from the spliceosome, makes splicing irreversible.

TLS Online TPP Program

#Id: 6678

#Unit 3. Fundamental Processes

Group II mitochondrial introns are excised by the same mechanism as nuclear pre-mRNAs via a lariat that is held together by a 2’–5’ bond.

TLS Online TPP Program

#Id: 6679

#Unit 3. Fundamental Processes

SR proteins are involved in exon definition in which they interact with U1 and U2AF forming commitment E complex.

TLS Online TPP Program

#Id: 6680

#Unit 3. Fundamental Processes

U11 base pairs with the 5’ splice sites; U12 base pairs with the branch point sequence near the 3’ splice site; and U4atac and U6atac provide analogous functions during the spliceosome assembly and activation of the spliceosome. 

U5 is same component in all splicing complexes

TLS Online TPP Program

#Id: 6681

#Unit 3. Fundamental Processes

Each snRNP contains a single snRNA (100-300 nt) and several (20) proteins. The U4 and U6 snRNPs are usually found together as a di-snRNP (U4/U6) particle.