DnaG primase associates transiently with the replication complex, and typically synthesizes a 10-base primer. Primers start with the sequence pppAG positioned opposite the sequence 3’–GTC-5’ in the template.

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#Id: 2744

#Unit 13. Methods in Biology

RAPDs behave as dominant markers and are recognized by the primer used for amplification.

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#Id: 2745

#Unit 13. Methods in Biology

RAPD bands are treated as independent loci;

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#Id: 2746

#Unit 13. Methods in Biology

A comparison between RFLP and RAPD markers for genome mapping in plan

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#Id: 2747

#Unit 13. Methods in Biology

RAPDs (Random amplified polymorphic DNAs) are obtained by using a PCR (polymerase chain reaction) equipment or a thermal cycler. 

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#Id: 2748

#Unit 13. Methods in Biology

The RAPD technique is based on PCR, which uses a DNA polymerase that is resistant to the high temperature needed for DNA denaturation; this permits the enzyme to be used repeatedly during the amplification process. 

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#Id: 2749

#Unit 13. Methods in Biology

RAPDs are more sensitive than RFLPs to experimental conditions making them more difficult to be consistent and reproducible.