Supplementary gene interaction (9:3:4)

Glume colour in millets

          P                                   Q

X                         Y                                   Z

Brown               purple                   red

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#Id: 863

#Unit 13. Methods in Biology

Thermal modulations are used in PCR for the functions of helicase and polymerase.

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#Id: 864

#Unit 13. Methods in Biology

In the PCR denaturation temperature calculated by using the Wallace rule, which is, Tm = 4 × (number of GC base pairs) + 2 × (number of AT base pairs).

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#Id: 865

#Unit 13. Methods in Biology

The size of a PCR product is mainly determined by the binding location of the PCR primers.

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#Id: 866

#Unit 13. Methods in Biology

The standard PCR does not efficiently amplify sequences much longer than about 3 kb.

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#Id: 867

#Unit 13. Methods in Biology

Size of the primer negatively affects the efficiency of PCR.

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#Id: 868

#Unit 13. Methods in Biology

PCR amplification is mathematically counted as 2n So 10 cycle leads to 1024 times amplification