The complete enzyme or holoenzyme in E. coli has a molecular weight of ,460 kD. The holoenzyme (a2bbws) can be separated into two components: the core enzyme (a2bbw) and the sigma factor (the s polypeptide), which is concerned specifically with promoter recognition.

TLS Online TPP Program

#Id: 7072

#Unit 3. Fundamental Processes

The charging of a tRNA with its cognate amino acid is a remarkably accurate process.

Experimental measurements indicate, for example, that at equal concentrations of isoleucine and valine, IleRS transfers ∼40,000 isoleucines to tRNAIle for every valine it so transfers.

TLS Online TPP Program

#Id: 7801

#Unit 2. Cellular Organization

An RNA helicase (Dbp5) located on the cytoplasmic side of the NPC removes NXF1 and NXT1 from the mRNA in a reaction that is powered by ATP hydrolysis.

TLS Online TPP Program

#Id: 8616

#Unit 2. Cellular Organization

Phosphorylation of T14 and Y15 in this G-loop

introduces negative charge that electrostatically interferes with the binding of ATP in the catalytic pocket.

TLS Online TPP Program

#Id: 9287

#Unit 6. System Physiology – Plant

TLS Online TPP Program

#Id: 10342

#Unit 4. Cell Communication and Cell Signaling