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TLS Online TPP Program

#Id: 6321

After binding to the rut site, rho uses its helicase activity, driven by ATP hydrolysis, to translocate along RNA until it reaches the RNA polymerase

#Unit 3. Fundamental Processes #Prokaryotic Transcription #Part B Pointers
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TLS Online TPP Program

#Id: 368

#Unit 2. Cellular Organization

Supercoiling is related to two properties of genome
1. Packaging 
2. Linking number
TLS Online TPP Program

#Id: 369

#Unit 2. Cellular Organization

DNA Packaging or genome packaging is expressed in terms of Packaging Ratio=Original size/packed size  
TLS Online TPP Program

#Id: 370

#Unit 2. Cellular Organization

Linking number is topological property which is expressed as  Total No. of Base Pair/Base pair per turn.
TLS Online TPP Program

#Id: 371

#Unit 2. Cellular Organization

How to calculate linking number?
TLS Online TPP Program

#Id: 372

#Unit 2. Cellular Organization

If Lk <Lk0 and DLk is <0 means negative, then the DNA is said to be “negatively supercoiled.”
TLS Online TPP Program

#Id: 373

#Unit 2. Cellular Organization

Conversely, if Lk >Lk0  and DLk >0 means positive, then the DNA is “positively supercoiled.”