Attenuator riboswitch (Leader sequence) region 1 is translated atleast till codon 10 and 11 in case of Low trp concentration because ribosomes get stalled due to low availability of charged tRNA-trp

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#Unit 1. Molecules and their Interaction Relevant to Biology

van’t Hoff plot is used measure rate of unfolding of proteins

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#Unit 1. Molecules and their Interaction Relevant to Biology

Techniques for Measuring Stability

Absorbance (e.g. Trp, Tyr) 

Fluorescence (Trp)-difference in emission max & intensity.

CD (far or near UV) - (2o or 3o) 

NMR 

DSC (calorimetry) 

Urea gradient gels - difference in the migrating rates between F and U.

Catalytic activity 

Chromophoric or fluorophoric probes/FRET 

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#Unit 1. Molecules and their Interaction Relevant to Biology

The Cp heat capacity of a defined system is the amount of heat (usually expressed in calories, kilocalories, or joules) needed to raise the system's temperature by one degree (usually expressed in Celsius or Kelvin).

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#Unit 1. Molecules and their Interaction Relevant to Biology

Thermal stability of protein can be calculated

TLS Online TPP Program

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#Unit 1. Molecules and their Interaction Relevant to Biology

TLS Online TPP Program

#Id: 8777

#Unit 1. Molecules and their Interaction Relevant to Biology

A.

1 - native

3 - MG

2, 4 - unfolded

B.

1 - native

3, 4 - MG

2 - unfolded