Fluorochrome-coupled monoclonal antibodies to the FLAG or myc epitopes can then be used to detect the recombinant protein in the cell.

Alexa- 488-labeled antibody -emit green light

Alexa-568-labeled antibody- emit red light

TLS Online TPP Program

#Id: 3961

#Unit 7. System Physiology – Animal

Conducting cells constitute tissues of the SA node, the atrial internodal tracts, the AV node, the bundle of His, & the Purkinje system.

TLS Online TPP Program

#Id: 3964

#Unit 7. System Physiology – Animal

Conducting cells are specialized muscle cells which function to rapidly spread action potentials over the entire myocardium.

TLS Online TPP Program

#Id: 3973

#Unit 7. System Physiology – Animal

The action potential spreads throughout the myocardium in the following sequence: SA node  Atrial internodal tracts and atria  AV node  Bundle of His  Purkinje system  Ventricles

TLS Online TPP Program

#Id: 3975

#Unit 7. System Physiology – Animal

TLS Online TPP Program

#Id: 3978

#Unit 7. System Physiology – Animal

Myocardial fibers have a resting membrane potential of approximately –90 mV.

TLS Online TPP Program

#Id: 3979

#Unit 7. System Physiology – Animal

The individual fibers are separated by membranes, but depolarization spreads radially (syncytium) because of the presence of gap junctions.