The telomerase uses the 3’–OH of the G+T telomeric strand as a primer for synthesis of tandem TTGGGG repeats.

 

TLS Online TPP Program

#Id: 2775

#Unit 13. Methods in Biology

Methylation interference assay Dimethyl sulphate (DMS) methylates purines on DNA strand. Methylate DNA prior to adding protein. Protein binding is prevented at methylated sites. After treatment and removal of protein of interest, treat with piperidine or NaOH which cuts strand at modified bases.

TLS Online TPP Program

#Id: 3509

#Unit 2. Cellular Organization

The homology between two genes can be plotted in the form of a dot matrix

TLS Online TPP Program

#Id: 6279

#Unit 3. Fundamental Processes

With s subunit, the holoenzyme worked equally well on both types of DNA

TLS Online TPP Program

#Id: 6417

#Unit 2. Cellular Organization

The trp operon is controlled by repression and attenuation, providing a two-stage response to progressively more stringent tryptophan starvation. But attenuation alone can provide robust regulation: other amino acid operons such as leu and his rely entirely on attenuation for their control. In the case of the leucine operon, its leader peptide has four adjacent leucine codons, and the histidine operon leader peptide has seven histidine codons in a row.

TLS Online TPP Program

#Id: 2342

#Unit 3. Fundamental Processes

The consensus sequence reflects preferred –10 and –35 regions, separated by the optimum spacing (17 bp).