TLS Online TPP Program

#Id: 2460

#Unit 13. Methods in Biology

During electrophoresis generation of heat is the major problem which leads to

ncreased rate of diffusion of sample and buffer ions.

Thermal instability which include denaturation of proteins.

A decrease of buffer viscosity.

Convection current leads mixing of separated samples

TLS Online TPP Program

#Id: 2461

#Unit 13. Methods in Biology

Electrophoresis can be performed in two environment 

In free solution - Moving boundary electrophoresis (A. G. Tiselius) & CE

n the medium  -  GEL ( Agarose & PAGE )

TLS Online TPP Program

#Id: 2462

#Unit 13. Methods in Biology

A. G. Tiselius (1937) Performed Moving boundary electrophoresis  in a U-shaped tube using Schlieren optics on both end for Measurements of the electrophoretic mobilities.

TLS Online TPP Program

#Id: 2463

#Unit 13. Methods in Biology

He received Nobel Prize in 1948 for developing technique for electrophoretic separation of substances.

TLS Online TPP Program

#Id: 2464

#Unit 13. Methods in Biology

Overall, there are three pillars electrophoresis

TLS Online TPP Program

#Id: 2465

#Unit 13. Methods in Biology

Medium - (buffer/liquid) - with a precise pH value and a constant ionic strength.