Bioactive brassinosteroid levels are also modulated by a variety of inactivation or catabolic reactions, including epimerization, oxidation, hydroxylation, sulfonation, and conjugation to glucose or lipids.

TLS Online TPP Program

#Id: 3128

#Unit 3. Fundamental Processes

The E. coli oriC contains 11 copies of the sequence GATC/CTAG which is a target for methylation at the N6 position of adenine by the Dam methylase enzyme.

TLS Online TPP Program

#Id: 3129

#Unit 3. Fundamental Processes

Before replication, the palindromic target site is methylated on the adenines of each strand.

TLS Online TPP Program

#Id: 3130

#Unit 3. Fundamental Processes

Replication inserts the normal (nonmodified) bases into the daughter strands. This generates hemimethylated DNA, in which one strand is methylated and one strand is unmethylated.

TLS Online TPP Program

#Id: 3131

#Unit 3. Fundamental Processes

Initiation may require full methylation of the Dam target sites in the origin, or it may be inhibited by hemimethylation of these sites.

TLS Online TPP Program

#Id: 3132

#Unit 3. Fundamental Processes

The GATC sites at the origin remain hemimethylated for -13 minutes after replication.

TLS Online TPP Program

#Id: 3133

#Unit 3. Fundamental Processes

Initiation at oriC requires the sequential assembly of a large protein complex on the membrane.