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The activity of rubisco is also regulated by light, but the enzyme itself does not respond to thioredoxin.
Two protons are released during the formation of the ternary complex rubisco–CO2–Mg2+, so activation is promoted by an increase in both pH and Mg2+ concentration.

#Unit 6. System Physiology – Plant #Regulation #Part B Pointers
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#Unit 13. Methods in Biology

Flow cytometry is based on light scattering and fluorescence 

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#Unit 13. Methods in Biology

Forward light scattering detect shape and side scattering detects granularity and complexity of the cell


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#Unit 13. Methods in Biology

Hydrodynamically focused stream separate cells in single file


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#Unit 13. Methods in Biology

2D Histogram 


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#Unit 13. Methods in Biology

Flow cytometry is majorly used with fluorescence dyes and attached antibodies for cells and dyes.

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#Unit 13. Methods in Biology

Tritiated (3H) Thymidine uptake used to assess Cell Division based on radioactivity measurement by scintillation counting. 

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