#Id: 2466
#Unit 13. Methods in Biology
Overall, there are three pillars electrophoresis
Current / voltage
Medium - (buffer/liquid) - with a precise pH value and a constant ionic strength.
Analytes - A compound, which is to be detected or assayed in a given sample.
Electrophoretic resolution mainly can be change by manipulating current and buffer system.
The higher the buffer concentration, the more electric power is applied, resulting in higher heat (joule effect) development.
Smiling Effect, Venetian Blind Effect & Electroendosmosis reduces the efficiency of electrophoresis.
