Each members of JAK family of kinases contains:

An N-terminal domain that binds to the receptor

A C-terminal kinase domain 

A middle pseudokinase domain

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#Id: 2725

#Unit 12. Applied Biology

Vectors For The Production Of Transgenic Plants

Plasmid vectors (Ti plasmid, Ri plasmid)

Plant virus vectors (Cauliflower Mosaic Virus, Gemini Viruses, Tobacco Mosaic Virus)

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#Id: 2726

#Unit 12. Applied Biology

Scorable reporter genes

The gene encoding green fluorescent protein (GFP) isolated from the jellyfish (Aequorea victoria) has emerged as a very useful scorable marker, and it does not require addition of any substrate, cofactor.

Gene uidA encodes the enzyme β-glucourinidase, which acts on the substrate X-gluc (5-Br-4 Cl-3-indolyl-β-D-glucuronide). The substrate is cleaved by the enzyme within plant cells (transformed/transgenic), and the product is converted into an insoluble blue precipitate at the site of enzyme activity.

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#Id: 2727

#Unit 12. Applied Biology

Selectable reporter genes

Selectable marker genes include those conferring resistance to methotrexate (dhfr, isolated from Tn7 or plasmid R67); the antibiotics kanamycin (nptII, encoding neomycin phosphotransfcrase) and hygromycin (hptIV, encoding hygromycin phosphotransferase, .isolated from E. coli);

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#Id: 2728

#Unit 12. Applied Biology

Which one of the following can be used as a selection marker for developing transgenic plants?

green fluorescent protein

β-glucuronidase

β-galactosidase

hygromycin phosphotransferase

 

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#Id: 2729

#Unit 12. Applied Biology

 Golden rice

The transgenic rice, popularly called "golden rice" contains good quantities of carotene, which gives the grains a golden colour. In one transgenic line, the β-carotene content was as high as 85% of the total carotenoids present in the grain.

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#Id: 2730

#Unit 12. Applied Biology