The highest concentrations of free auxin in the living plant are in the apical meristems of shoots and in young leaves because these are the primary sites of auxin synthesis. 

TLS Online TPP Program

#Id: 7089

#Unit 3. Fundamental Processes

IF1 prevents tRNAs from binding to the portion of the small subunit that will become part of the A-site.

†IF2 is a GTPase that interacts with three key components of the initiation machinery: the small subunit, IF1, and the charged initiator tRNA (fMet-tRNAi f-Met). By interacting with these components, IF2 facilitates the association of fMet-tRNAi f-Met with the small subunit and prevents other charged tRNAs from associating with the small subunit.

† IF3 binds to the small subunit and blocks it from reassociating with a large subunit. Because initiation requires a free small subunit, the binding of IF3 is critical for a new cycle of translation. 

TLS Online TPP Program

#Id: 7090

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 7091

#Unit 3. Fundamental Processes

binding by a ribosome, many prokaryotic ORFs contain a short sequence upstream (on the 5’ side) of the start codon called the ribosome binding site (RBS). This element is also referred to as a polypurine stretch Shine–Dalgarno sequence.

TLS Online TPP Program

#Id: 7092

#Unit 3. Fundamental Processes

It is complementary to a highly conserved sequence close to the 3’ end of 16S rRNA.

The rRNA sequence is the hexamer:

3’. . . U C C U C C . . . 5’

TLS Online TPP Program

#Id: 7093

#Unit 3. Fundamental Processes

 5’-AUGA-3’ is sequence between overlapping ORFs is known as translational coupling.

TLS Online TPP Program

#Id: 7094

#Unit 3. Fundamental Processes