#Id: 2779
#Unit 13. Methods in Biology
General structure and component of a chromatographic system
A stationary phase – typically an aqueous buffer with sufficient ionic strength to maintain a soluble protein.
A column -
An injector system: To deliver test samples to the top of the column in a reproducible manner.
A detector with data acquisition - To give a continuous record of the presence of the analytes in the eluate as it emerges from the column.
A fraction collector: For collecting the separated analytes for further biochemical studies.
