KDEL receptor, located mainly in the cis-Golgi network and in both COPII and COPI vesicles, binds proteins bearing the KDEL sorting signal and returns them to the ER

#Id: 748

#Unit 3. Fundamental Processes

Incorrect base pairing leads to dramatically lower rates of nucleotide addition called as kinetic proofreading

#Id: 749

#Unit 3. Fundamental Processes

A common method to monitor synthesis of new DNA is Incorporation Assays.

#Id: 750

#Unit 3. Fundamental Processes

rNTPs are present at approximately 10-fold higher concentration in the cell, they are incorporated at a rate that is more than 1000-fold lower than dNTPs.

#Id: 751

#Unit 3. Fundamental Processes

The palm domain is composed of a b sheet and contains the primary elements of the catalytic site.

#Id: 752

#Unit 3. Fundamental Processes

DNA polymerase binds two divalent metal ions (typically Mg2+ orZn2+) that alter the chemical environment around the correctly base-paired dNTP and the 3’-OH of the primer

#Id: 753

#Unit 3. Fundamental Processes

One metal ion generate a 3’O- that is primed for the nucleophilic attack of the a-phosphate of the incoming dNTP.