The palm domain is composed of a b sheet and contains the primary elements of the catalytic site.

TLS Online TPP Program

#Id: 8068

#Unit 13. Methods in Biology

TLS Online TPP Program

#Id: 8069

#Unit 13. Methods in Biology

A photoelastic modulator (PEM) is then used to convert the linearly polarized light into circularly polarized light.

TLS Online TPP Program

#Id: 8070

#Unit 13. Methods in Biology

An optically active chiral compound will absorb the two directions of circularly polarized light by different amounts

TLS Online TPP Program

#Id: 8073

#Unit 13. Methods in Biology

In a chiral medium the molar absorptivity of LCP and RCP light are different so they will be absorbed by the medium in different amounts. 

TLS Online TPP Program

#Id: 8074

#Unit 13. Methods in Biology

Differential absorption of the two circularly polarized lights resulted in elliptically polarized light and CD is represented in terms of molar ellipticity(θ).

θ = 3298    ϵ

TLS Online TPP Program

#Id: 8075

#Unit 13. Methods in Biology

β-sheets shows a negative bands at 218nm and a positive one at 196nm.

Random coils has a positive band at 212nm and a negative one around 195nm.