If weight of enzyme is given use molar conversion (wt/mol. Wt)

TLS Online TPP Program

#Id: 2808

#Unit 13. Methods in Biology

Reversed-phase liquid chromatography form of liquid chromatography, which has many similarities with hydrophobic interaction chromatography (HIC).

TLS Online TPP Program

#Id: 2809

#Unit 13. Methods in Biology

In RPLC the stationary phase is non-polar and the mobile phase relatively polar, hence the name reversed-phase

TLS Online TPP Program

#Id: 2810

#Unit 13. Methods in Biology

In RPLC stationary phase most commonly used is in the bonded-phase form, in which alkylsilane groups are chemically attached to silica. Butyl (C4), octyl (C8) and octadecyl (C18) silane groups are most commonly used.

TLS Online TPP Program

#Id: 2811

#Unit 13. Methods in Biology

In RPLC mobile phase is commonly water or aqueous buffers, methanol, acetonitrile or tetrahydrofuran, or mixtures of them.

TLS Online TPP Program

#Id: 2812

#Unit 13. Methods in Biology

 In RPLC the organic solvent is referred to as an organic modifier.

TLS Online TPP Program

#Id: 2813

#Unit 13. Methods in Biology

Reversed-phase liquid chromatography differs from most other forms of chromatography in that the stationary phase is essentially inert and only non-polar (hydrophobic) interactions are possible with analytes.