To nucleate the assembly of branched actin filaments, Arp2/3 needs to be activated by a nucleation promoting factor (NPF)

TLS Online TPP Program

#Id: 9767

#Unit 6. System Physiology – Plant

When an Arabidopsis leaf is exposed to bacteria on the leaf surface, or to the MAMP flg22, the stomatal apertures closed by LRR receptor-like kinase FLS2, as well as a heterotrimeric G protein.

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#Id: 5055

#Unit 3. Fundamental Processes

DnaA then acts at three A-T–rich 13-bp tandem repeats located on the left side of oriC.

TLS Online TPP Program

#Id: 2757

#Unit 13. Methods in Biology

Linkers have the following two applications: creation of cohesive 'ends

on blunt~ended DNA fragments, and 

on fragments having unmatched or undefined sequences in their protruding ends. 

TLS Online TPP Program

#Id: 8406

#Unit 2. Cellular Organization

Vertebrate skeletal muscle contraction, also depends on set of specialized accessory proteins that are closely associated with the actin thin filaments:

a) Tropomyosin

b) Troponin

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#Id: 2775

#Unit 13. Methods in Biology

Methylation interference assay Dimethyl sulphate (DMS) methylates purines on DNA strand. Methylate DNA prior to adding protein. Protein binding is prevented at methylated sites. After treatment and removal of protein of interest, treat with piperidine or NaOH which cuts strand at modified bases.