Nurturing Life Sciences
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#Unit 3. Fundamental Processes
hydroxylating agent hydroxylamine (NH2OH) induces only G:C → A:T transitions not reversal.
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When DNA is treated with hydroxylamine, the amino group of cytosine is hydroxylated.
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X rays, gamma rays, and cosmic rays is ionizing radiation and ultraviolet light is nonionizing radiation.
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1 roentgen unit is a quantity of X ray that produces 2.083X109 ion pairs in one cubic centimeter of air at 0C and a pressure of 760 mm of mercury.
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The maximum absorption of UV by DNA is at a wavelength of 254 nm. Maximum mutagenicity also occurs at 254 nm.
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Two major products of UV absorption by pyrimidines (thymine and cytosine) are pyrimidine hydrates and pyrimidine dimers.