Nurturing Life Sciences
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#Unit 3. Fundamental Processes
The subunits of RNA Polymerases
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Template and transcript within the RNA polymerase elongating complex
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Core enzyme without the s subunit could not transcribe viral DNA, yet had no problems with highly nicked calf thymus DNA
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With s subunit, the holoenzyme worked equally well on both types of DNA
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Consensus Sequences
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The carboxy-terminal domain of the a subunit (aCTD) recognizes the Up-element (where present), whereas s regions 2 and 4 recognize the –10 and –35 regions