#Id: 2612

#Unit 13. Methods in Biology

Our on-going research indicates that RLMRACE, PPM-RACE, and qRT-PCR are very effective in the verification of sequences of miRNA targets obtained by Degradome sequencing. 

#Id: 2613

#Unit 13. Methods in Biology

The protocol for RLM-RACE, PPMRACE, and qRT-PCR is rapid, effective, cheap, and can be completed within 2–3 day.

#Id: 2614

#Unit 13. Methods in Biology

To more accurately validate the predicted target genes of miRNAs and exactly determine the cleavage sites within the targets, an integrated strategy comprising RLM-RACE, Poly(A) Polymerase-Mediated (PPM)-RACE, and qRT-PCR was developed.

#Id: 2615

#Unit 13. Methods in Biology

In plants, the perfect complementarity between most miRNAs and their targets enables the accurate predictions of their targets, while slicing of the targeted mRNAs facilitates target validation through RNA Ligase-Mediated (RLM)-Rapid Amplification of cDNA Ends (RACE) method.

#Id: 2616

#Unit 13. Methods in Biology

Type I restriction endonucleases are complex endonucleases and have recognition sequences of about 15 bp; 

#Id: 2617

#Unit 13. Methods in Biology

Type I restriction endonucleases cleave the DNA about 1000 bp away from the 5'-end of the sequence "TCA" located within the recognition site.