The G1 cyclin-CDK complex (Cln3-CDK in yeast) stimulates the formation of G1/S phase cyclin-CDKs, which initiate bud formation, centrosome duplication, and DNA replication.

#Id: 2761

#Unit 13. Methods in Biology

A gel mobility shift assay, is a method for detecting DNA–protein interaction relies on the fact that a small DNA has a much higher mobility in gel electrophoresis than the same DNA does when it is bound to a protein. 

#Id: 9057

#Unit 4. Cell Communication and Cell Signaling

glutamate (E200) and arginine (R209) participate in binding of cAMP, which causes a conformational change in the loop that prevents binding of the loop to the C subunit.

#Id: 397

#Unit 2. Cellular Organization

Nucleosomal DNA wraps around the histone protein 1.65 times, so create a writhe of –1.65 and so DLk should be -1.65. But when the change in linking number associated with each nucleosome was measured, the number was lower than this, approximately –1.2 for each nucleosome added. This discrepancy is referred to as the “Nucleosome Linking Number paradox,”

#Id: 2599

#Unit 13. Methods in Biology

TaqMan assay also applicable in analyzing variations in the allele or deletion 

of base substitution.

#Id: 1467

#Unit 4. Cell Communication and Cell Signaling