The efficiency of PCR can be calculated by using Y = X (1+ E)n X is number of templates, E is mean PCR efficiency, n is number is PCR cycle. 

 

TLS Online TPP Program

#Id: 9056

#Unit 4. Cell Communication and Cell Signaling

TLS Online TPP Program

#Id: 9057

#Unit 4. Cell Communication and Cell Signaling

glutamate (E200) and arginine (R209) participate in binding of cAMP, which causes a conformational change in the loop that prevents binding of the loop to the C subunit.

TLS Online TPP Program

#Id: 9058

#Unit 4. Cell Communication and Cell Signaling

Epinephrine --> Gαs-GTP-->Adenylyl cyclase-->cAMP-->PKA

TLS Online TPP Program

#Id: 9059

#Unit 4. Cell Communication and Cell Signaling

PKA always phosphorylates a serine or threonine residue that is located within the common sequence motif: X-Arg-(Arg/Lys)-X-(Ser/Thr)-Φ

TLS Online TPP Program

#Id: 9060

#Unit 4. Cell Communication and Cell Signaling

Increased cAMP; PKA active, increased glycogen breakdown, and decreased glycogen synthesis

TLS Online TPP Program

#Id: 9061

#Unit 4. Cell Communication and Cell Signaling

The GPK in skeletal muscle is a huge protein with multiple subunits:

Kinase subunit contains the catalytic activity that activates GP 

Regulatory subunits is the ubiquitous protein calmodulin