Following securin degradation and a decrease in mitotic CDK activity, separase cleaves the Scc1 subunit, breaking the cohesin circles.

TLS Online TPP Program

#Id: 2614

#Unit 13. Methods in Biology

To more accurately validate the predicted target genes of miRNAs and exactly determine the cleavage sites within the targets, an integrated strategy comprising RLM-RACE, Poly(A) Polymerase-Mediated (PPM)-RACE, and qRT-PCR was developed.

TLS Online TPP Program

#Id: 2615

#Unit 13. Methods in Biology

In plants, the perfect complementarity between most miRNAs and their targets enables the accurate predictions of their targets, while slicing of the targeted mRNAs facilitates target validation through RNA Ligase-Mediated (RLM)-Rapid Amplification of cDNA Ends (RACE) method.

TLS Online TPP Program

#Id: 2616

#Unit 13. Methods in Biology

Type I restriction endonucleases are complex endonucleases and have recognition sequences of about 15 bp; 

TLS Online TPP Program

#Id: 2617

#Unit 13. Methods in Biology

Type I restriction endonucleases cleave the DNA about 1000 bp away from the 5'-end of the sequence "TCA" located within the recognition site.

TLS Online TPP Program

#Id: 2618

#Unit 13. Methods in Biology

Type II restriction endonucleases are remarkably stable and induce cleavage either, in most cases, within or immediately outside their recognition sequences.

TLS Online TPP Program

#Id: 2619

#Unit 13. Methods in Biology

More than 350 different type II endonucleases with over 100 different recognition sequences are known.