Conjugation

Direct transfer of genetic material from one bacterium to another.

Two bacteria lie close together and a connection forms between them. 

After conjugation, crossing overtakes place between homologous sequences

In conjugation, DNA is transferred only from donor to recipient, with no reciprocal exchange of genetic material. 

TLS Online TPP Program

#Id: 2286

#Unit 3. Fundamental Processes

Transcription that begins at promoters is specific, directed by the s-subunit

TLS Online TPP Program

#Id: 2287

#Unit 3. Fundamental Processes

How tightly does core enzyme v. holoenzyme bind DNA?

TLS Online TPP Program

#Id: 2288

#Unit 3. Fundamental Processes

The effect of temperature on the dissociation of the polymerase–T7 DNA complex. 

TLS Online TPP Program

#Id: 2289

#Unit 3. Fundamental Processes

higher temperature favors tighter binding between RNA polymerase holoenzyme and T7 DNA

TLS Online TPP Program

#Id: 2290

#Unit 3. Fundamental Processes

RNA polymerase holoenzyme binds DNA loosely at first

a. Binds at promoter initially

b. Scans along the DNA until it finds one

TLS Online TPP Program

#Id: 2291

#Unit 3. Fundamental Processes

Isomerization, does not require energy derived from ATP hydrolysis and is instead the result of a spontaneous conformational change in the DNA– enzyme complex to a more energetically favorable form.