Phosphorylation by MEK at Y-185 and T-183 in activation loop of MAP kinase leads to a marked conformational change & promotes MAP kinase activation and dimerization.

TLS Online TPP Program

#Id: 2868

#Unit 13. Methods in Biology

The absorption of UV light by nucleic acids arises from n ® p* and p ® p* transitions of the purine and pyrimidine bases that occur between 260nm and 275 nm.

TLS Online TPP Program

#Id: 2869

#Unit 13. Methods in Biology

The absorption spectra of the bases in polymers are sensitive to pH and greatly influenced by electronic interactions between bases.

TLS Online TPP Program

#Id: 2870

#Unit 13. Methods in Biology

The characteristic absorption parameter for the sample is the extinction coefficient a, yielding the correlation I= I0e-ad.

TLS Online TPP Program

#Id: 2871

#Unit 13. Methods in Biology

The ratio T= I/I0 is called transmission.

TLS Online TPP Program

#Id: 2872

#Unit 13. Methods in Biology

Algebraic transformation of the exponential correlation into an expression based on the decadic logarithm yields the law of Beer–Lambert:

where [d] = 1cm, [c] = 1moldm-3, and [e] = 1dm3mol-1 cm1. e is the molar absorption   coefficient.

where [d] = 1cm, [c] = 1moldm-3, and [e] = 1dm3mol-1 cm1. e is the molar absorption coefficient.

TLS Online TPP Program

#Id: 2873

#Unit 13. Methods in Biology

Absorbance of the sample is displayed on the spectrophotometer.