For many aphids, EBF is the principal constituent of the aphid alarm pheromone, and the constitutive production of EBF by plants could hold promise in the control of specific aphid pests

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#Id: 4454

#Unit 13. Methods in Biology

Negative staining- heavy metal coats the grid but is excluded from the regions where the sample has adhered.

TLS Online TPP Program

#Id: 4455

#Unit 13. Methods in Biology

Commonly used heavy metals

Uranyl acetate

Osmium tetraoxide 

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#Id: 4456

#Unit 13. Methods in Biology

Metal shadowing- thin film of platinum is coated from a fixed angle. it provides information about the three-dimensional topology of the sample.

TLS Online TPP Program

#Id: 4457

#Unit 13. Methods in Biology

Scanning electron microscopy (SEM) allows investigators to view the surfaces of unsectioned metal-coated specimens

TLS Online TPP Program

#Id: 4458

#Unit 13. Methods in Biology

Molecules in the metal coating are excited and release secondary electrons that are focused onto a scintillation detector

TLS Online TPP Program

#Id: 4459

#Unit 13. Methods in Biology

Immunoelectron microscopy is used for localizing proteins in thin sections at the electron microscope level.