LRR motif also found in the extracellular domain of several P/MAMP

TLS Online TPP Program

#Id: 734

#Unit 3. Fundamental Processes

Initiation of replication in E.Coli at oriC starts with formation of a complex that ultimately requires six proteins: DnaA, DnaB, DnaC, HU, gyrase, and SSB

TLS Online TPP Program

#Id: 735

#Unit 3. Fundamental Processes

The minimal origin is defined by the distance between the outside members of the 13-mer and 9-mer repeats.

TLS Online TPP Program

#Id: 736

#Unit 3. Fundamental Processes

The four 9-bp consensus sequences on the right side of oriC provide the initial binding sites for DnaA-ATP

TLS Online TPP Program

#Id: 737

#Unit 3. Fundamental Processes

A two-state assembly model explains melting of 13mer during initiation

TLS Online TPP Program

#Id: 738

#Unit 3. Fundamental Processes

To identify DNA that is in the process of replicating, DNA derived from dividing cells is first cut with a restriction enzyme and separated on a 2D agarose gel.

TLS Online TPP Program

#Id: 739

#Unit 3. Fundamental Processes

DNA strand synthesis requires the four deoxynucleoside triphosphates—dGTP, dCTP, dATP, and dTTP in equal in vivo concentration