TLS Online TPP Program

#Id: 2613

#Unit 13. Methods in Biology

The protocol for RLM-RACE, PPMRACE, and qRT-PCR is rapid, effective, cheap, and can be completed within 2–3 day.

TLS Online TPP Program

#Id: 9788

#Unit 6. System Physiology – Plant

Mutations in the EDS1 (SA synthesis) (ENHANCED DISEASE SENSITIVITY 1) gene eliminate race‐specific resistance mediated by the TIR‐NB‐LRR class of R proteins

TLS Online TPP Program

#Id: 5568

#Unit 7. System Physiology – Animal

Assume in addition that the pumps in the thick ascending limb can pump 100 mOsm/kg of Na+ & Cl– from the tubular fluid to the interstitium, increasing interstitial osmolality to 400 mOsm/kg of H2O 

TLS Online TPP Program

#Id: 7611

#Unit 5. Developmental Biology

Polyspermy occurs more frequently in mouse eggs bearing mutant ZP2 that cannot be cleaved by ovastacin

TLS Online TPP Program

#Id: 790

#Unit 3. Fundamental Processes

Sequences of Rho dependent transcription termination

1. Rho binds a rut site. 

2. The rho factor then tracks along the RNA until it catches up to RNA polymerase. 

3. When the RNA polymerase reaches the termination site, rho first freezes the structure of the polymerase and then invades the exit channel to destabilize the enzyme, causing it to release the RNA. 

4. Pausing by the polymerase at the site of termination allows time for rho factor to translocate to the hybrid stretch.