The Wnt-Ca2+ pathway is initiated by Frizzled receptors activating a classical G protein-coupled signaling pathway.

TLS Online TPP Program

#Id: 1849

#Unit 3. Fundamental Processes

Fluorochrome-coupled monoclonal antibodies to the FLAG or myc epitopes can then be used to detect the recombinant protein in the cell.

Alexa- 488-labeled antibody -emit green light 

Alexa-568-labeled antibody- emit red light

TLS Online TPP Program

#Id: 1850

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 1851

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 1852

#Unit 3. Fundamental Processes

The CTD contains a series of repeats of the heptapeptide sequence: Tyr-Ser-Pro-Thr-Ser-Pro-Ser. 

TLS Online TPP Program

#Id: 1853

#Unit 3. Fundamental Processes

There are 27 of these repeats in the yeast Pol II CTD, 32 in the worm Caenorhabditis elegans, 45 in the fly Drosophila, and 52 in humans. 

YSPTSPS repeats in CTD corresponds to the complexity of the genome.

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#Id: 1854

#Unit 3. Fundamental Processes

Class II promoters have two parts:

Core promoter having 4 elements

Upstream promoter element