After a meal, when blood glucose rises, circulating insulin binds to insulin receptors on various cell types and reduces blood glucose levels by:

a. fusion of intracellular vesicles containing GLUT4 glucose transporters with the plasma membrane.

b. stimulation of the conversion of glucose to glycogen.

c. inhibition of glucose synthesis from smaller molecules.

Which of the following combination is correct?

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#Question id: 16315

#Unit 7. System Physiology – Animal

Eustress

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#Question id: 7059

#Unit 5. Developmental Biology

A Drosophila gene active in both males and females, but whose RNA transcript is spliced in a sex-specific manner to produce sex-specific transcription factors-

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#Question id: 26899

#Unit 10. Ecological Principles

Which statement is correct about Temperate Grassland?

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#Question id: 32272

#Unit 2. Cellular Organization

Which of the following extended disordered proteins most abundant in the central part of the nuclear pore complex?

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#Question id: 13089

#Unit 13. Methods in Biology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 2’s plasmid is;