Arrange the following events in the proper order in which they occur during transduction of the EGF signal:

a. transphosphorylation of the EGF receptor

b. dissociation of GDP from Ras                   

c. dissociation of Ras from Sos         

d. binding of GRB2 to the EGF receptor      

e. binding of GTP to Ras  

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#Question id: 23161

#Unit 13. Methods in Biology

A classically used fluorescent dye which emits a orange light is?

TLS Online TPP Program

#Question id: 23162

#Unit 13. Methods in Biology

Match the fluorescent dyes in column I with emission light in column II

Column I                                                                          Column II

1. Texas                                                                            a. far red

2. Fluorescein                                                                   b. red

3. Rhodamine                                                                   c. blue 

4. Cy5                                                                               d. green

Which of the following option comprises all correct matches?

TLS Online TPP Program

#Question id: 23163

#Unit 13. Methods in Biology

Out of the given below alexa dyes which one will emit in red light range?

TLS Online TPP Program

#Question id: 23164

#Unit 13. Methods in Biology

Out of the given below alexa dyes which one will emit in Blue light range?

TLS Online TPP Program

#Question id: 23165

#Unit 13. Methods in Biology

For visualizing two proteins which are localized in nucleus through fluorescence microscopy which two sets of dyes can be best used for clear visual?

TLS Online TPP Program

#Question id: 23166

#Unit 13. Methods in Biology

To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.

1. FITC

2. GFP

3. Fluorescein

4. Alexa 568

Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?