Pull-down assays using proteins that ONLY bind to the GTP-bound version of the small G-protein can be used to detect when small G proteins like Rac1 have been activated by various growth factors.  You were given two lysates, one having been treated with platelet-derived growth factor (PDGF) and the other not treated with PDGF. This method is similar to performing co-IPs, so based on that knowledge, which of the following steps is erroneous?

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#Question id: 33388

#Unit 1. Molecules and their Interaction Relevant to Biology

At what pH where polyglu will readily forms an α-helix.

TLS Online TPP Program

#Question id: 33389

#Unit 1. Molecules and their Interaction Relevant to Biology

At what pH where polygly will readily forms an α-helix.

TLS Online TPP Program

#Question id: 33390

#Unit 1. Molecules and their Interaction Relevant to Biology

why Gly is most conserved in type II B turns, its due to

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#Question id: 33391

#Unit 1. Molecules and their Interaction Relevant to Biology

α-Turn 

a. Consists of 5 amino acids, generally termed as i, 1+1, i+2, i+3 and i+4. 

b. H-bond is seen between i and i+4 (First and Fifth residue).

c. The conformation of i+1, i+2 and i+3 are crucial

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#Question id: 33392

#Unit 1. Molecules and their Interaction Relevant to Biology

For γ-Turns, which is of the following statement is correct

a. Consists of 5 amino acids

b. H-bond is seen between i and i+3

c. The conformation of i+1 is crucial 

d. dihedral angles of residue i+1 are (70, -60)

which is of the following statements are correct

TLS Online TPP Program

#Question id: 33393

#Unit 1. Molecules and their Interaction Relevant to Biology

In case of Beta Hairpin