TLS Online TPP Program

#Question id: 1240


Pull-down assays using proteins that ONLY bind to the GTP-bound version of the small G-protein can be used to detect when small G proteins like Rac1 have been activated by various growth factors.  You were given two lysates, one having been treated with platelet-derived growth factor (PDGF) and the other not treated with PDGF. This method is similar to performing co-IPs, so based on that knowledge, which of the following steps is erroneous?

#Unit 4. Cell Communication and Cell Signaling
  1. To assay for levels of active Rac1, PAK1-beads (the protein that only binds GTP-bound Rac1-conjugated to beads similar to Nickel beads) are added to each of the lysates described above and incubated on a rocking platform (on ice). 

  2. The tubes are vortexed well, loading buffer is added, and the samples are heated to aid in protein denaturation.

  3. The samples are loaded and analyzed by SDS-polyacrylamide gel electrophoresis, then the proteins are transferred to a membrane. 

  4. The membrane (a.k.a. blot) will be probed with the anti-Rac antibody.

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TLS Online TPP Program

#Question id: 851

#Unit 1. Molecules and their Interaction Relevant to Biology

Methods is the Rosetta program, formulated by David Baker. To satisfy the program’s computational needs, a volunteer network of ∼100,000 computers, known as Rosetta@home, provides the 500,000 or so hours of processing time required to generate a structure;

TLS Online TPP Program

#Question id: 852

#Unit 1. Molecules and their Interaction Relevant to Biology

.________ enzyme revealed the relation between the amino acid sequence of a protein and its conformation.

TLS Online TPP Program

#Question id: 853

#Unit 1. Molecules and their Interaction Relevant to Biology

In the presence of a large excess of _______ the disulfides (cystines) are fully converted into sulfhydryls (cysteines).

TLS Online TPP Program

#Question id: 854

#Unit 1. Molecules and their Interaction Relevant to Biology

Many conditions including type 2 diabetes, Alzheimer  disease, Huntington disease and parkinson disease are associated with the misfolding mechanism a soluble protein that is normally secreted from the cell is secreted in the missfold state and converted into an insoluble extracellular fibre the diseases are correctly referred to as

TLS Online TPP Program

#Question id: 855

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of the following interactions make a large contribution to the stability of protein structures

TLS Online TPP Program

#Question id: 856

#Unit 1. Molecules and their Interaction Relevant to Biology

Protein folding is not a completely random, trial-and-error process, This problem was first pointed out by