TLS Online TPP Program

#Question id: 1240


Pull-down assays using proteins that ONLY bind to the GTP-bound version of the small G-protein can be used to detect when small G proteins like Rac1 have been activated by various growth factors.  You were given two lysates, one having been treated with platelet-derived growth factor (PDGF) and the other not treated with PDGF. This method is similar to performing co-IPs, so based on that knowledge, which of the following steps is erroneous?

#Unit 4. Cell Communication and Cell Signaling
  1. To assay for levels of active Rac1, PAK1-beads (the protein that only binds GTP-bound Rac1-conjugated to beads similar to Nickel beads) are added to each of the lysates described above and incubated on a rocking platform (on ice). 

  2. The tubes are vortexed well, loading buffer is added, and the samples are heated to aid in protein denaturation.

  3. The samples are loaded and analyzed by SDS-polyacrylamide gel electrophoresis, then the proteins are transferred to a membrane. 

  4. The membrane (a.k.a. blot) will be probed with the anti-Rac antibody.

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TLS Online TPP Program

#Question id: 1696

#Unit 4. Cell Communication and Cell Signaling

TCR recognition of peptide-MHC class II depends on:

TLS Online TPP Program

#Question id: 18755

#Unit 13. Methods in Biology

Once the double stranded molecule with the mutation is introduced into E. coli for replication, how many types of molecules are produced?

TLS Online TPP Program

#Question id: 12114

#Unit 10. Ecological Principles

Researchers have found that a region of the canary forebrain shrinks during the nonbreeding season and enlarges when breeding season begins. This annual enlargement of brain tissue is probably associated with the annual

TLS Online TPP Program

#Question id: 10413

#Unit 6. System Physiology – Plant

Following are certain statements are related to the effect of red and far-red light;
a) under a dense canopy much of the red light is absorbed by plant pigments, resulting in transmittance of mostly far-red light
b) Pr (red) has highest photon flux at 680 nm while Pfr (far-red) has highest photon flux at 730nm
c) Pfr far-red light–absorbing form, which is considered to be the physiologically active form of phytochrome
d) R:FR ratio is strongly affected by the presence of a canopy because chlorophyll absorbs far-red but not red light
Which one of the combinations of above statements is incorrect?

TLS Online TPP Program

#Question id: 4476

#Unit 3. Fundamental Processes

Regulatory elements include promoter proximal elements, upstream activator sequences (UASs), enhancers, and a series of other element