TLS Online TPP Program

#Question id: 15266


In DNA isolation, which of the following enzyme remove/digest proteins in presence of SDS or chelating agent

#Unit 13. Methods in Biology
  1. Proteinase K
  2. chymotrypsin
  3. ficin,
  4.  papain
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TLS Online TPP Program

#Question id: 11330

#Unit 10. Ecological Principles

Bio geographers have noted that the tropics have more terrestrial species than temperate regions. Which of the following is NOT a plausible explanation for this pattern?

TLS Online TPP Program

#Question id: 20409

#Unit 3. Fundamental Processes

Introns may more freely accumulate point substitutions and other changes due to 

TLS Online TPP Program

#Question id: 13192

#Unit 10. Ecological Principles

In the schematic below, the left panel represents climatic zones occupied by two different biomes, X and Y, along gradients of temperature and precipitation. The right panel depicts the expected species-area relationships of these two biomes. From the figures below, which of the following are most likely to be true?


TLS Online TPP Program

#Question id: 4096

#Unit 3. Fundamental Processes

The lambda repressor binds as a dimer to critical sites on the bacteriophage lambda genome to keep the lytic genes turned off, which allows the bacteriophage lambda genome to be maintained as a silent resident in the bacterial genome. Each molecule of the repressor consists of an N-terminal DNA-binding domain and a C-terminal dimerization domain. Upon induction (for example, by irradiation with ultraviolet light), the genes for lytic growth are expressed, bacteriophage lambda progeny are produced, and the bacterial cell lyses to release the viral progeny. Induction is initiated by cleavage of the lambda repressor at a site between the DNA-binding domain and the dimerization domain. In the absence of bound repressor, RNA polymerase initiates transcription of the lytic genes, triggering lytic growth.

A. Binding as monomers will be sufficiently weak that they do not compete with the binding of RNA polymerase. As a result, the genes for lytic growth will be turned on.

B. Binding as monomers will be sufficiently strong that they will compete with the binding of RNA polymerase. As a result, the genes for lysogenic growth will be turned on

C. The affinity of the dimeric lambda repressor for its binding site is the sum of all the interactions made by each DNA-binding domain. An individual DNA-binding domain will make just half the contacts and provide just half the binding energy as the dimer.

D. The affinity of the dimeric lambda repressor for its binding site is the sum of all the interactions made by each DNA-binding domain. An individual DNA-binding domain will make just double the contacts and provide just double the binding energy as the dimer.

Given that the number (concentration) of DNA-binding domains is unchanged by cleavage of the repressor, which of above outcomes will be possible?

TLS Online TPP Program

#Question id: 31111

#Unit 2. Cellular Organization

Arp2/3 needs to be activated by interacting with a nucleation promoting factor (NPF). Although there are many different NPFs, The major NPF family is characterized by the presence of a region called: