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#Question id: 15417

Sequence of amphibian lens induction postulated by experiments on embryos of the frog Xenopus laevis ; EXCEPT

#Unit 5. Developmental Biology
  1. Unidentified inducers (possibly from the foregut endoderm and cardiac mesoderm) cause the synthesis of the Otx2 transcription factor in the head ectoderm during the late neurula stage.
  2. As the neural folds rise, inducers from the anterior neural plate (including the region that will form the retina) induce Pax6 expression in the anterior ectoderm that can form lens tissue.
  3. As the neural folds rise, inducers from the anterior neural plate (including the region that will form the retina) induce Pax6 expression in the anterior ectoderm that can form lens tissue.
  4. The optic vesicle secretes BMP and FGF family paracrine factors, which induce the synthesis of the Sox transcription factors and initiate observable lens formation
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TLS Online TPP Program

#Question id: 19840

#Unit 13. Methods in Biology

Match the following source of thermostable DNA polymerase with proofreading (3’−5’ exonuclease) activity;

                 DNA Polymerase

                        Sources

 

 A- Tma

 

i) Pyrococcus furiosus

 

 B- Deep VentTM

 

ii) Pyrococcus woesi

 

 C- Tli

 

iii)  Thermotoga maritima

 

 D- Pfu

 

iv) Pyrococcus sp.

 

 E- Pwo

 

v) Thermococcus litoralis

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#Question id: 19841

#Unit 13. Methods in Biology

Which of the following is true regarding Reverse transcriptase ?
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#Question id: 19842

#Unit 13. Methods in Biology

Primer-Dimer leads to the formation of ___
a) Inter-complemetary primers
b) Hybrid 
c) Amplicons
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#Question id: 19843

#Unit 13. Methods in Biology

Real time PCR analyzed various steps such as__
a) Denaturation
b) Annealing
c) Phosphorylation
d) Amplification
e) Methylation
f) Extension
Which of the following steps are correctly analyzed by real time PCR?
TLS Online TPP Program

#Question id: 19844

#Unit 13. Methods in Biology

Match the following column, which correctly represent the qPCR done by three methods and measure such as;

                  COLUMN I

                    COLUMN II

A) SYBR green

i) Annealing

B) Taqman Assay

ii) Denaturation

C) Molecular beacon

iii) pH

 

iv) Amplification

 

v) Extension

TLS Online TPP Program

#Question id: 19845

#Unit 13. Methods in Biology

SYBR Green qPCR method is based on____I_______ with the _____II______ relationship.